Key points for ELISA
Dilution of condensed reagents - standard solution, Labeled antibody solution
Washing of microplate - Dilution of washing buffer, High bank absorbance, Low or too much colouration, Peeling off of capture antibody
Shaking of microplate - Low colouration, Big variation, Poor sensitivity, 600 ~ 1,200rpm, 10sec.x3 times
Assay circumstance - Room temp(20~25 Celsius), Humidity(>30%), Airflow(0,4m/sec.>), Level, Avoidance of direct sunlight
Precaution to the safety - Gloves, Glasses, Mask, Laboratory Coats, 1M Sulphuric Acid, Assay Sample handling, Disposal of materials after assays, Storage of kits and term of validity
Instrumentation - Beaker-Graduated cylinder, Pipette - Tips, Vortex-type mixer, Thermometer-Hygrometer, Timer, Microplate reader(450nm, 620nm), Test tubes, Test rucks, others
Temperature adaptation - Temperature Adaptation Time, For partial use, TMB
Handling of pipette - Touch&Go, Pre-wetting, Co-washing
We would like to inform you that the highly sensitive Rat Thyroid Stimulating Hormone ELISA Kits from Shibayagi is available again and is quickly taking over the biological sphere.
These assays were unfortunately discontinued in 2012, but now Shibayagi released the new and improved version of those assays.
It is the most sensitive Rat TSH ELISA on the market. It uses 2 2 monoclonal antibodies directed to the α- and β-subunits of the TSH molecule which is a heterodimer.
Male CD (SD) rats, 9 weeks of age, fasting, TSH values: 3,26~4,65 ng/ml , n=5
Male CD (SD) rats, 7 months of age, non-fasting, TSH values: 2,50~4,04 ng/ml, n=5
Male CD (SD) rats, 9 weeks of age, non-fasting, thyroidectomized, TSH values: 8,09~11,9 ng/ml , n=5